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Does whole body vibration exercise improve oxidative stress markers in women with fibromyalgia? BJMBR
Santos,J.M.; Mendonça,V.A.; Ribeiro,V.G.C.; Tossige-Gomes,R.; Fonseca,S.F.; Prates,A.C.N.; Flor,J.; Oliveira,A.C.C.; Martins,J.B.; Garcia,B.C.C.; Leite,H.R.; Figueiredo,P.H.S.; Bernardo-Filho,M.; Lacerda,A.C.R..
The objective of this study was to investigate the effect of whole body vibration (WBV) exercise on oxidative stress markers in a group of women with fibromyalgia (FM) compared to a group of healthy women (CT). Twenty-one women diagnosed with FM and 21 age- and weight-matched healthy women were enrolled the study. Plasma oxidative stress markers (primary outcomes) were evaluated at rest and after WBV, and included thiobarbituric acid reactive substances (TBARS), iron reduction capacity (FRAP), superoxide dismutase antioxidant enzymes activity (SOD), and catalase (CAT). At rest, the FM group had higher TBARS (P<0.001) and FRAP (P<0.001), and lower CAT (P=0.005) compared to the CT. In the CT group, the WBV had no effect on TBARS (P=0.559) and FRAP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Whole body vibration; Chronic disease; Oxidative stress; Fibromyalgia.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800609
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Effect of oral ingestion of an extract of the herb Uncaria tomentosa on the biodistribution of sodium pertechnetate in rats BJMBR
Moreno,S.R.F.; Silva,A.L.C.; Diré,G.; Honeycut,H.; Carvalho,J.J.; Nascimento,A.L.; Pereira,M.; Rocha,E.K.; Oliveira-Timóteo,M.; Arnobio,A.; Olej,B.; Bernardo-Filho,M.; Caldas,L.Q.A..
The aim of the present study was to determine the effect of the oral ingestion of an extract of the herb Uncaria tomentosa (cat's claw) on the biodistribution of the radiobiocomplex sodium pertechnetate (Na99mTcO4) in rats. The animals (male Wistar rats, 2 months old, 180-220 g), were treated (1 mL) with an U. tomentosa extract (32 mg/mL, N = 5) or 0.9% NaCl solution (control, N = 5) for 7 days. After this period, Na99mTcO4 (3.7 MBq, 0.3 mL) was injected through the ocular plexus and after 10 min the rats were killed, the organs isolated and counted in a well-gamma counter. A significant (P < 0.05) alteration in Na99mTcO4 uptake i) from 0.57 ± 0.008 to 0.39 ± 0.06 %ATI/organ (P < 0.05) and from 0.57 ± 0.17 to 0.39 ± 0.14 %ATI/g (P < 0.05) was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Uncaria tomentosa; Biodistribution; Sodium pertechnetate; Radiobiocomplex.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100010
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In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements BJMBR
Ripoll-Hamer,E.; De-Paula,E.F.; Freitas,L.C.; Pereira,M.J.S.; Fonseca,L.M.; Gutfilen,B.; Carvalho,J.J.; Pôrto,L.C.M.S.; Bernardo-Filho,M..
In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Radioautography; Radiopharmaceuticals; Blood elements.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014
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Protection of plasmid DNA by a Ginkgo biloba extract from the effects of stannous chloride and the action on the labeling of blood elements with technetium-99m BJMBR
Moreno,S.R.F.; Freitas,R.S.; Rocha,E.K.; Lima-Filho,G.L.; Bernardo-Filho,M..
Ginkgo biloba extract (EGb) is a phytotherapeutic agent used for the treatment of ischemic and neurological disorders. Because the action of this important extract is not fully known, assays using different biological systems need to be performed. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and used in nuclear medicine. The labeling depends on a reducing agent, usually stannous chloride (SnCl2). We assessed the effect of different concentrations of EGb on the labeling of blood constituents with Tc-99m, as sodium pertechnetate (3.7 MBq), and on the mobility of a plasmid DNA treated with SnCl2 (1.2 µg/ml) at room temperature. Blood was incubated with EGb before the addition of SnCl2 and Tc-99m. Plasma (P) and RBC were separated and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Ginkgo biloba extract; Blood element labeling; Technetium-99m; Stannous chloride; Plasmid DNA.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200015
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